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  • 94

    Structured Review

    Alomone Labs acc
    Acc, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 53 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+cav3+1/Anti-CACNA1G+(CaV3%2E1)+Antibody/pm42025169-537-26-24
    Average 94 stars, based on 53 article reviews
    acc - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Wnt/β-Catenin Signaling Causes Sinoatrial Node Dysfunction by Inhibition of T-type Calcium Current
    Article Snippet: 2 Aims: Calcium currents are critical for the excitation-contraction coupling of atrial and ventricular 2 myocytes and for the pacemaker function of the sinoatrial node (SAN).. The Wnt/β-catenin signaling is 3 activated in heart failure that is frequently associated with SAN dysfunction.. However, it is unknown if 4 Wnt/β-catenin signaling regulates calcium currents in cardiomyocytes and affects SAN function.

    Incubation:

    Article Title: Wnt/β-Catenin Signaling Causes Sinoatrial Node Dysfunction by Inhibition of T-type Calcium Current
    Article Snippet: 2 Aims: Calcium currents are critical for the excitation-contraction coupling of atrial and ventricular 2 myocytes and for the pacemaker function of the sinoatrial node (SAN).. The Wnt/β-catenin signaling is 3 activated in heart failure that is frequently associated with SAN dysfunction.. However, it is unknown if 4 Wnt/β-catenin signaling regulates calcium currents in cardiomyocytes and affects SAN function.

    Article Title: Estrogen receptor beta in lateral habenula mediates antidepressant effects of estrogen in postpartum-hormone-withdrawal-induced depression
    Article Snippet: .. The membranes were incubated with mouse anti-ERα (1:250, Santa Cruz), mouse anti-ERβ (1:250, Santa Cruz), rabbit anti-GPR30 (1:500, Abcam), rabbit anti-Kir4.1 intracellular peptide (1:1000, Alomone labs), rabbit anti-Cav3.1 (1:500, Alomone labs) mouse anti-NR1(1:3000, Millipore), mouse anti-α-tubulin (1:5000, Sigma) and mouse anti-GAPDH-HRP antibodies (1:5000, KangChen Bio-tech Inc.) at 4 °C overnight. .. After rinses with TBST buffer, membranes were then incubated with goat anti-rabbit-HRP(1:2000, Sigma) or goat anti-mouse-HRP antibody (1:2000, Biorad).

    Article Title: Upregulation of T-type voltage-dependent Ca²⁺ channels in activated hepatic stellate cells promotes liver fibrosis in metabolic dysfunction-associated steatohepatitis.
    Article Snippet: 1 Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabedori Mizuhoku, Nagoya 467-8603, Japan Abstract Activation of hepatic stellate cells (HSCs) is a pivotal event in the development of liver fibrosis, a pathological process that precedes cirrhosis and hepatocellular carcinoma and for which effective therapies remain limited.. The molecular mechanisms underlying HSC activation, particularly the ion channels that regulate cytosolic Ca2+ signaling that promote fibrogenesis, remain incompletely understood.. In this study, the expression and functional roles of Ca2+ channels in human and mouse HSCs were investigated using quantitative real-time PCR, Western blotting, whole-cell patch-clamp electrophysiology, Ca2+ imaging, and histological analyses.

    Article Title: Effects of progesterone on T-type-Ca 2+ -channel expression in Purkinje cells
    Article Snippet: The next day, after thorough washing with PBS (2 × 2 minutes plus 2 × 10 minutes), the samples were incubated with goat anti-mouse tetramethylrhodamine (TRITC) antibody (1:1000 in PBS T5393, MilliporeSigma; RRID: AB_261699) at room temperature for 2 hours. .. After washing with PBS (2 × 2 minutes plus 2 × 10 minutes), samples were additionally incubated with the following antibodies against T-type-Ca 2+ -channels at 4°C overnight: rabbit anti-Cav3.1 (1:1500 in PBS, Alomone Labs, Jerusalem, Israel; ACC21, RRID: AB_2039779); rabbit anti-Cav3.2 (1:1500 in PBS, Alomone Labs; ACC25, RRID: AB_2039781) or rabbit anti-Cav3.3 (1:1000 in PBS, Alomone Labs; ACC009, RRID: AB_2039783). .. On the 3 rd day, after thorough washing with PBS, incubation of the secondary donkey anti-rabbit antibody was followed at room temperature for 2 hours (1:1000 in PBS, Thermo Fisher Scientific; AF488, RRID: AB_2535792).

    Article Title: Estrogen receptor beta in lateral habenula mediates antidepressant effects of estrogen in postpartum-hormone-withdrawal-induced depression.
    Article Snippet: .. The membranes were incubated with mouse anti-ERα (1:250, Santa Cruz), mouse anti-ERβ (1:250, Santa Cruz), rabbit anti-GPR30 (1:500, Abcam), rabbit anti-Kir4.1 intracellular peptide (1:1000, Alomone labs), rabbit anti-Cav3.1 (1:500, Alomone labs) mouse anti-NR1(1:3000, Millipore), mouse anti-α-tubulin (1:5000, Sigma) and mouse anti-GAPDH-HRP antibodies (1:5000, KangChen Biotech Inc.) at 4 °C overnight. .. After rinses with TBST buffer, membranes were then incubated with goat anti-rabbit-HRP(1:2000, Sigma) or goat antimouse-HRP antibody (1:2000, Biorad).

    Saline:

    Article Title: Upregulation of T-type voltage-dependent Ca²⁺ channels in activated hepatic stellate cells promotes liver fibrosis in metabolic dysfunction-associated steatohepatitis.
    Article Snippet: 1 Department of Molecular and Cellular Pharmacology, Graduate School of Pharmaceutical Sciences, Nagoya City University, 3-1 Tanabedori Mizuhoku, Nagoya 467-8603, Japan Abstract Activation of hepatic stellate cells (HSCs) is a pivotal event in the development of liver fibrosis, a pathological process that precedes cirrhosis and hepatocellular carcinoma and for which effective therapies remain limited.. The molecular mechanisms underlying HSC activation, particularly the ion channels that regulate cytosolic Ca2+ signaling that promote fibrogenesis, remain incompletely understood.. In this study, the expression and functional roles of Ca2+ channels in human and mouse HSCs were investigated using quantitative real-time PCR, Western blotting, whole-cell patch-clamp electrophysiology, Ca2+ imaging, and histological analyses.

    Control:

    Article Title: Cav3.1 is a leucine sensor in POMC neurons mediating appetite suppression and weight loss
    Article Snippet: .. In brief, 33.3 μl beads per sample were washed with 1 mL TBS with 1% Triton X-100 (TBST) then blocked with 500 μl TBST with 1μg/ml BSA for 20 min at 4°C with rotation, followed by incubating with 1 μg N-terminal anti-Cav3.1 (Alomone Labs, Cat# ACC-021) (or 1 μg rabbit IgG as a control) in 500 μl lysis buffer with 1 μg/ml BSA at 4°C with rotation for 1 hr. ..

    Lysis:

    Article Title: Cav3.1 is a leucine sensor in POMC neurons mediating appetite suppression and weight loss
    Article Snippet: .. In brief, 33.3 μl beads per sample were washed with 1 mL TBS with 1% Triton X-100 (TBST) then blocked with 500 μl TBST with 1μg/ml BSA for 20 min at 4°C with rotation, followed by incubating with 1 μg N-terminal anti-Cav3.1 (Alomone Labs, Cat# ACC-021) (or 1 μg rabbit IgG as a control) in 500 μl lysis buffer with 1 μg/ml BSA at 4°C with rotation for 1 hr. ..



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